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Abha A Saini

from Collegeville, PA
Age ~54

Abha Saini Phones & Addresses

  • 2 Norsham Way, Collegeville, PA 19426 (484) 831-5252 (301) 216-9695
  • Montgomery, PA
  • 105 Mill Grove Dr #105, Norristown, PA 19403
  • 639 Mill Grove Dr #639, Norristown, PA 19403
  • Audubon, PA
  • 5101 River Rd, Bethesda, MD 20816 (301) 652-7780
  • North Potomac, MD

Industries

Pharmaceuticals

Resumes

Resumes

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Abha Saini

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Location:
Greater Philadelphia Area
Industry:
Pharmaceuticals

Publications

Us Patents

Human Brain Endothelial Cells And Growth Medium And Method For Expansion Of Primitive Cd34+Cd38-Bone Marrow Stem Cells

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US Patent:
6642049, Nov 4, 2003
Filed:
Dec 3, 1999
Appl. No.:
09/452855
Inventors:
John P. Chute - San Francisco CA
Abha A. Saini - Collegeville PA
Dennis J. Chute - Los Angeles CA
Thomas A. Davis - Newton PA
Assignee:
The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:
C12N 500
US Classification:
435373, 435368, 435384, 435385
Abstract:
A novel co-culture system using human brain endothelial cells (HUBEC) which promotes the expansion of human CD34 CD38 cells consistent with the PMVEC system is disclosed. HUBEC were isolated from cadaveric donors, passed in primary culture, cloned and found to be Von Willebrand Factor positive. Cultivation of purified bone marrow CD34 cells on HUBEC monolayers supplemented with GM-CSF+IL-3+IL-6+SCF+flt-3 ligand caused a 14. 5-fold increase in total cells, an 6. 6-fold increase in CD34 cells, and, most remarkably, a 440-fold increase in CD34 CD38 cells after 7 days. Further, CFU-GM production increased 15. 1-fold, BFU-E increased 8-fold, and CFU-Mix increased 5. 2-fold. Optimal generation was dependent upon the continued presence of exogenous supplied cytokines. Moreover, we found that non-brain human endothelial cells isolated from the same donors supported neither the expansion nor the maintenance of human CD34 CD38 cells.
Abha A Saini from Collegeville, PA, age ~54 Get Report